Optimization of ELISA buffers volume for CD-based microfluidic biosensors

Microfluidic devices are strong technological tools in analyzing the proteins and have been designed for development of immunoassays. Enzyme-linked immunosorbent assay (ELISA) is a standard immunoassay technique for Dengue virus detection. In comparison to the macroscale devices, decreased consumption of reagents is needed in microfluidic designs with microscale fluidic networks. In order to achieve optimized consumption of ELISA buffers for microfluidic devices, we have reduced the volumes on 4mm×4mm dimension Polymethylmetacrylate (PMMA) pieces, set into the wells of ELISA 96-well plate. The general concept of this work was to minimize the ELISA buffers´ volume for application in microfluidic diagnostic devices. We accomplished the test by six different volumes of buffers on small pieces of PMMA polymer as the most common polymeric material for production of biosensor devices. Conventional ELISA has been considered as control. We demonstrate that 40μl is an optimized volume of ELISA buffers, effective for microfluidic devices.