Aptamer immobilization on MWCNT-modified SGFET for detection of HIV-1 Tat protein

Author

Fatin, M.F. ; Ruslinda, A. Rahim ; Md Arshad, M.K. ; Norhafizah, S. ; Farehanim, M.A. ; Ayub, R.M. ; Hashim, U.

Author_Institution

Inst. of Nano Electron. Eng., Univ. Malaysia Perlis, Kangar, Malaysia

fYear

2014

Firstpage

840

Lastpage

844

Abstract

A solution gate field effect transistor (SGFET) has been fabricated for the detection of HIV-1 Tat protein. Functionalized carboxylated multiwalled carbon nanotube (COOH-MWCNT) has been deposited on the FET to act as amplification layer and immobilization site for aptamer. Aptamer has been a very reliable probe for detection of protein with high specificity. In this paper, we demonstrated the immobilized aptamer on MWCNT-modified SGFET. The bond formation and surface morphology of MWCNT were examined using FTIR and FESEM, respectively. The fabricated device shows the current decreases due to resistance increased after every immobilization step. The implementation of aptamer as a recognition element is promising for HIV-1 Tat protein detection.

Keywords

biosensors; carbon nanotubes; cellular biophysics; coating techniques; electrochemical sensors; field effect transistors; materials preparation; microorganisms; molecular biophysics; polymers; proteins; COOH-MWCNT deposition; FESEM technique; FET amplification layer; FTIR technique; Fourier transform infrared radiation technique; HIV-1 tat protein detection; HIV-1 tat protein recognition; MWCNT bond formation; MWCNT surface morphology; MWCNT-modified SGFET; SGFET fabrication; aptamer immobilization site; field emission scanning electron microscopy technique; functionalized carboxylated nanotube; high-specificity protein detection; human immunodeficiency virus; immobilized aptamer; multiwalled carbon nanotube; protein detection specificity; protein recognition element; reliable probe; solution gate field effect transistor fabrication; Biosensors; DNA; Electrodes; Logic gates; Protein engineering; Proteins; Surface treatment;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Sciences (IECBES), 2014 IEEE Conference on

Type

conf

DOI

10.1109/IECBES.2014.7047629

Filename

7047629