Identification model and properties of the membrane transport mechanism of the microbial enzyme catalysis of 1,3-propanediol based on robustness analysis

This paper consider a microbial continuous process. Hybrid nonlinear systems are developed for describing the dynamics of the process under three possible transmembrane transport modes of the goal product 1,3-propanediol. The established model is also based on the assumptions that the transmembrane transport mode of the substrate glycerol is a combination of active and passive transport, and that the specific growth rate of the microorganism is not inhibited by 3-hydroxypropionaldehyde. Parameter identification model is established with the quantitative definition of the given system´s robustness as performance index. The model was proved identifiable. The contributions of this work could provide theoretical reference for numerical inference of the membrane transport mechanism.