Nanomanipulation for the measurement of single-cell shear force using a self-sensitive cantilever

Author

Hashimoto, Shigetaka ; Iwata, Futoshi

Author_Institution

Dept. of Mech. Eng., Shizuoka Univ., Hamamatsu, Japan

fYear

2014

Firstpage

7

Lastpage

11

Abstract

This paper describes a nanomanipulator system based on an atomic force microscope (AFM) for the measurement of the shear force of a single adhesion cell on a substrate. The shear force is measured based on the deflection of a self-sensitive cantilever that is employed for simple configuration of the AFM manipulator. The shear force behavior of a single cell detaching from the substrate is observed. The shear force of the cell increases with increasing size of the cell. Slight differences in the shear force of the single cell under different conditions can be successfully observed. The shear force of cells on a polystyrene dish treated for adhesion cells is higher than that of cells on a glass substrate dish. With respect to different buffers, the shear force of cells in the tested buffer containing Ca²⁺ was significantly higher than that of cells in the buffer with no added Ca²⁺.

Keywords

adhesion; atomic force microscopy; biomechanics; biomedical measurement; cantilevers; cellular biophysics; force measurement; AFM manipulator; atomic force microscopy; glass substrate dish; nanomanipulator system; polystyrene dish; self-sensitive cantilever; single adhesion cell; single-cell shear force measurement; Adhesives; Force; Force measurement; Glass; Manipulators; Substrates; Wires; AFM; manipulator; self-sensitive cantilever; shear force; single cell;

fLanguage

English

Publisher

ieee

Conference_Titel

Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO), 2014 International Conference on

Type

conf

DOI

10.1109/3M-NANO.2014.7057314

Filename

7057314