Title :
Multilevel Regulation and Translational Switches in Synthetic Biology
Author :
Kopniczky, Margarita B. ; Moore, Simon J. ; Freemont, Paul S.
Author_Institution :
Dept. of Med., Imperial Coll. London, London, UK
Abstract :
In contrast to the versatility of regulatory mechanisms in natural systems, synthetic genetic circuits have been so far predominantly composed of transcriptionally regulated modules. This is about to change as the repertoire of foundational tools for post-transcriptional regulation is quickly expanding. We provide an overview of the different types of translational regulators: protein, small molecule and ribonucleic acid (RNA) responsive and we describe the new emerging circuit designs utilizing these tools. There are several advantages of achieving multilevel regulation via translational switches and it is likely that such designs will have the greatest and earliest impact in mammalian synthetic biology for regenerative medicine and gene therapy applications.
Keywords :
RNA; cellular biophysics; gene therapy; molecular biophysics; proteins; gene therapy; mammalian synthetic biology; multilevel regulation; post transcriptional regulation; protein; regenerative medicine; ribonucleic acid; synthetic genetic circuits; translational regulators; translational switches; Circuit synthesis; Gene expression; Logic gates; Protein engineering; Proteins; RNA; Synthetic biology; Biomedical engineering; circuit design; logic devices; protein; ribonucleic acid (RNA); riboswitch; synthetic biology; translation;
Journal_Title :
Biomedical Circuits and Systems, IEEE Transactions on
DOI :
10.1109/TBCAS.2015.2451707
