Sensitive and Specific Protein Sensing Using Single-Mode Tapered Fiber Immobilized With Biorecognition Molecules

Author

Mustapha Kamil, Y. ; Abu Bakar, M.H. ; Mustapa, M.A. ; Yaacob, M.H. ; Syahir, A. ; Mahdi, M.A.

Author_Institution

Res. Centre of Excellence for Wireless & Photonic Networks, Univ. Putra Malaysia, Serdang, Malaysia

Volume

7

Issue

6

fYear

2015

Firstpage

1

Lastpage

9

Abstract

We examine and demonstrate a biosensor using single-mode tapered fiber that has been immobilized with biorecognition molecules to sense targeted proteins. Interaction of evanescent waves with the external medium surrounding the tapered region produces an interferometric-patterned spectrum, which shifts correspondingly to any changes of refractive index (RI) in the external medium. The proposed setup managed to obtain an RI sensitivity and concentration sensitivity of 2526.8 nm/RIU and 20.368 nm/μM, respectively, which, to our knowledge, is highly sensitive when compared with previous studies. The dynamic performance, good specificity, and high sensitivity of the proposed method highlight an immensely beneficial choice for immunological diagnostics.

Keywords

biochemistry; biosensors; chemical sensors; fibre optic sensors; light interferometry; molecular biophysics; proteins; refractive index; biorecognition molecules; evanescent wave-external medium interaction; immunological diagnostics; interferometric-patterned spectrum; protein sensing; refractive index sensitivity; single-mode tapered fiber immobilization; Biosensors; Optical fiber sensors; Optical fiber testing; Optical fibers; Refractive index; Sensitivity; Tapered microfiber; biophotonics instrumentation; biosensors; refractive-index sensor; tapered microfiber;

fLanguage

English

Journal_Title

Photonics Journal, IEEE

Publisher

ieee

ISSN

1943-0655

Type

jour

DOI

10.1109/JPHOT.2015.2490486

Filename

7297801