Biological particle manipulation: an example of Jurkat enrichment

Author

Altinagac, Emre ; Kizil, Huseyin ; Trabzon, Levent

Author_Institution

Dept. of Nanosci. & Nanoengineering, Istanbul Tech. Univ., Istanbul, Turkey

Volume

10

Issue

10

fYear

2015

Firstpage

550

Lastpage

553

Abstract

Dielectrophoresis-based separation of microparticles and living cells are studied with computational and experimental methods. The flow behaviours of particles are modelled and simulated using COMSOL Multiphysics 4.3a software (COMSOL Ltd). Lab-on-a-chip devices having a titanium (Ti) interdigitated electrode layer on a glass substrate and a PDMS microchannel are fabricated to investigate the most effective design for separating particles based on their sizes. Polystyrene particles in different diameters (3.2 and 9.8 μm) are used in the experiments. Green polystyrene particles from red ones are fully separated in a current design. Enrichment of Jurkat cells having 10.1 average diameter in phosphate buffer solution (PBS) medium is achieved.

Keywords

bioMEMS; cellular biophysics; electrodes; electrophoresis; lab-on-a-chip; microfluidics; polymers; COMSOL Multiphysics 4.3a software; Jurkat cell enrichment; PDMS microchannel fabrication; biological particle manipulation; dielectrophoresis-based separation; glass substrate; green polystyrene particle; lab-on-a-chip device; living cell; microparticle flow behaviour; microparticle flow simulation; microparticle separation; polydimethylsiloxane; red polystyrene particle; size 3.2 mum; size 9.8 mum; titanium-interdigitated electrode layer;

fLanguage

English

Journal_Title

Micro Nano Letters, IET

Publisher

iet

ISSN

1750-0443

Type

jour

DOI

10.1049/mnl.2015.0185

Filename

7312569