The counting of mycorrhizal fungi spores using fuzzy mathematical morphology

The mathematical morphology presents a systematic model to extract geometrical characteristics of images using morphological operators, which transforms the original image into another, using a third image called structural element. The fuzzy mathematical morphology extends the morphological operators to grayscale and coloured images using the fuzzy logic, where the definition of the operators are defined using the concepts of implications and fuzzy conjunctions, specifically, the implications and conjunctions of Lukasiewicz. In this paper it was proposed a counting method of mycorrhizal fungi spores which are derived from mycorrhizas, a symbiotic association between a fungus and a plant, using fuzzy morphological operators. The counting of these spores was done manually using corrugated plate and the aid of a stereoscopic microscope.