Alginate gel electro-writing for 3D cell printing

This paper presented a method of electro deposition of calcium alginate for cell 3D Printing. Alginate gel (calcium alginate) was introduced as printing material by electro-depositing. An ITO glass was set as cathode and a metal needle was used as anode. A DC current was applied to the electrodes. The calcium alginate was generated on the surface of anode, which is immersed in mixed solution of sodium alginate and CaCO₃. The thickness of gel, which is produced on the anode surface, increases along with enlargement of current. At last, we mixed cells in the sodium alginate solution (1%) with suspended CaCO₃ particles (0.5%) to observe the growth situation of yeast cells and osteoblast. The experiment result shown the call will be alive in 3 days.