The measurement of cell viability based on temporal bag of words for image sequences

The measurement of cell viability is an important and challenging topic in the cellular property researches. In this paper, we have presented a novel framework to measure the cell viability from both contour deformation and cytoplasm streaming for image sequences. The framework is distinguished by two aspects: First, we construct the appearance change field on the basis of the displacement field in order to more completely evaluate the streaming of protoplasm. Second, Temporal Bag of Words (TBoW) is introduced to capture the variation of cell viability across the temporal dimension. Experiments show that the proposed method is effective to quantize the cell viability.