Cell Extraction Method from Time-Series Image of Phase Contrast Microscope

In this study, we propose cell extraction method for solving the problem that previous research and common method cause extra segmentation and recognize single cell as plural cells. Proposed method consists of three steps. First, we separate the cell area from background of the image. Second, we classify the cell area into two groups by the size of area. Third, we extract cells from cell area classified each groups. As a result, we can decrease the number of extra segmentation of cell in the image.