Title :
Seeing an explosive way of NSF/SNAP-mediated SNARE-complex disassembly using single-molecule measurements
Author :
Je-Kyung Ryu;Duyoung Min;Sang-Hyun Rah;Haesoo Kim;Reinhard Jahn;Tae-Young Yoon
Author_Institution :
National Creative Research Initiative Center for Single-Molecule Systems Biology, KAIST, Daejeon 305-701, South Korea
Abstract :
N-ethylmaleimide-sensitive factor (NSF) and alpha soluble NSF attachment protein (α-SNAP) disassemble the SNAP receptor (SNARE) complex for recycling of the SNARE proteins. Using single-molecule fluorescence and force spectroscopy, we found that NSF appears to use a "spring-loaded" mechanism.
Keywords :
"Proteins","Fluorescence","Yttrium","Substrates","Microscopy","Magnetic separation","Magnetic resonance imaging"
Conference_Titel :
Lasers and Electro-Optics Pacific Rim (CLEO-PR), 2015 11th Conference on
DOI :
10.1109/CLEOPR.2015.7376355
