Different methods for obtaining of some bio-composite materials with dental use

In modern dentistry and maxillofacial surgery, for replacement and reconstruction of bone tissue, different materials are used. The demand for bone substitute materials has increased in recent years due to increased number of road and rail accidents, loss of teeth due to poor nutrition and stress, the emergence of new trauma and tumors, and bone reconstructive surgery due to development. The aim of this work was to create new nanomaterials from bio-wastes. Two methods are performed for hydroxyapatite extraction from bovine bones: hydrothermal process with water at subcritical and supercritical conditions (200-400°C) and microwave technology. Type I collagen from pig skin was extracted under stirring, using pepsin and acetic acid 0.5M at 4°C. A collagen-hydroxyapatite composite material for dental uses has been made by physical mixing of components in an ultrasonic field. The obtained bio-nanomaterials were characterized by TEM-EDX, X-ray diffraction and FT-IR techniques.