High definition immunosensing by fluorescence anisotropy measurement

A high definition immunosensor for antigen and nonspecific protein was developed. Immunosensor has been studied for decades as highly sensitive and specific sensor device against any of the antigen materials. However no solid device has successfully discriminated the target antigen in mixture solution of nonspecific proteins. This is simply because large amount of physical adsorption of nonspecific molecule intervenes the specific detection of antigen molecule. In this study, fluorescence anisotropy measurement method is employed for direct monitoring of antibody molecule motion, and antigen antibody binding was detected with no interference of nonspecific adsorption. With spot antibody immobilization technique, simultaneous multi-channel detection was demonstrated, with sensitivity of fg/mL.