Active control of cell with microbubbles for cellular immunotherapy by acoustic force

Recently immunotherapy using therapeutic cells has attracted attention. Because the cells injected in human body disperse through the blood stream, there is a problem that small amount reaches to the diseased area. So we intend for in vivo cell delivery system. Microbubbles were attracted on the surface of cells for dynamic control of cells under ultrasound emission. The cells and microbubbles were observed under fluorescent excitation to be observed their behavior in a thin channel, where the cells were induced to boundary of the channel. Furthermore, mean velocity of the cells increased with maximum sound pressure of the ultrasound, where the highest velocity of 2.2 mm/s was confirmed when the maximum sound pressure of 500 kPa-pp was applied. Through the experiments there is a possibility of delivery of in vivo cells.