High-resolution imaging of living biological specimens

Author

Murakami, M. ; Tsuboi, T. ; Kawata, Y. ; Terakawa, S. ; Egami, C. ; Sugihara, O. ; Okamoto, N. ; Tsuchimori, M. ; Watanabe, O.

Author_Institution

Fac. of Eng., Shizuoka Univ., Japan

Volume

1

fYear

2001

fDate

15-19 July 2001

Abstract

We present a near-field optical microscope without the use of a probe for illumination or detection or scattering of the optical fields. In our system, the optical fields near specimens are converted to the topographical change of a photosensitive film, and then the topography is detected with an atomic force microscope. Urethane-urea copolymer films are used for the conversion material from the optical fields to the topography. We succeeded in imaging of biological specimens with the resolution of subwavelength.

Keywords

atomic force microscopy; biological techniques; image resolution; near-field scanning optical microscopy; optical images; surface topography; atomic force microscope; euglena gracilis; fine structures; high-resolution imaging; intensity distribution; living biological specimens; near-field optical microscope; nerve growth factor treated cell; optical fields; paramecium; pheochromocytoma cells; photosensitive film; subwavelength resolution; topographical change; urethane-urea copolymer films; very fast phenomena; Atom optics; Atomic force microscopy; Biomedical optical imaging; High-resolution imaging; Lighting; Optical films; Optical microscopy; Optical scattering; Probes; Surfaces;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics, 2001. CLEO/Pacific Rim 2001. The 4th Pacific Rim Conference on

Conference_Location

Chiba, Japan

Print_ISBN

0-7803-6738-3

Type

conf

DOI

10.1109/CLEOPR.2001.967787

Filename

967787