Title :
High-resolution imaging of living biological specimens
Author :
Murakami, M. ; Tsuboi, T. ; Kawata, Y. ; Terakawa, S. ; Egami, C. ; Sugihara, O. ; Okamoto, N. ; Tsuchimori, M. ; Watanabe, O.
Author_Institution :
Fac. of Eng., Shizuoka Univ., Japan
Abstract :
We present a near-field optical microscope without the use of a probe for illumination or detection or scattering of the optical fields. In our system, the optical fields near specimens are converted to the topographical change of a photosensitive film, and then the topography is detected with an atomic force microscope. Urethane-urea copolymer films are used for the conversion material from the optical fields to the topography. We succeeded in imaging of biological specimens with the resolution of subwavelength.
Keywords :
atomic force microscopy; biological techniques; image resolution; near-field scanning optical microscopy; optical images; surface topography; atomic force microscope; euglena gracilis; fine structures; high-resolution imaging; intensity distribution; living biological specimens; near-field optical microscope; nerve growth factor treated cell; optical fields; paramecium; pheochromocytoma cells; photosensitive film; subwavelength resolution; topographical change; urethane-urea copolymer films; very fast phenomena; Atom optics; Atomic force microscopy; Biomedical optical imaging; High-resolution imaging; Lighting; Optical films; Optical microscopy; Optical scattering; Probes; Surfaces;
Conference_Titel :
Lasers and Electro-Optics, 2001. CLEO/Pacific Rim 2001. The 4th Pacific Rim Conference on
Conference_Location :
Chiba, Japan
Print_ISBN :
0-7803-6738-3
DOI :
10.1109/CLEOPR.2001.967787
