Title :
Stem cell separation using thermoresponsive copolymer brushes having cationic charge
Author :
Kenichi Nagase;Yuri Hatakeyama;Tatsuya Shimizu;Katsuhisa Matsuura;Masayuki Yamato;Naoya Takeda;Teruo Okano
Author_Institution :
Institute of Advanced Biomedical Engineering and Science, Tokyo Women´s Medical University, 8-1 Kawadacho, Shinjuku, 162-8666, Japan
Abstract :
For developing new stem cell separation tool, we have prepared thermoresponsive cationic copolymer brush, poly(N-isopropylacrylamide-co-N,N-dimethylaminopropylacrylamide-co-N-tert-butylacrylamide) (IPAAm-co-DMAPAAm-co-tBAAm) on glass substrates through surface-initiated atom transfer radical copolymerization (ATRP). Using the prepared thermoresponsive cationic copolymer brush, mesenchymal stem cell separation was performed simply by changing temperature.
Keywords :
"Brushes","Temperature measurement","Glass","Substrates","Fluorescence","Lead","Polymers"
Conference_Titel :
Micro-NanoMechatronics and Human Science (MHS), 2015 International Symposium on
DOI :
10.1109/MHS.2015.7438250
