Vapor phase SAW immunoassay sensors

In this paper we present the results of a series of experiments on vapor phase SAW biosensors. For these experiments the sensor component was a ST-X Quartz resonator with a center frequency of approximately 250MHz. Antibodies were attached to the electrodes on the device surface via a protein-A crosslinker. The SAW biosensor response was monitored and a baseline frequency shift was observed when the analyte being presented was the antigen for the immobilized antibody. Since all the analytes being used in these experiments were fluorescent we were able to observe tightly bound analytes using a confocal laser scanning microscope (CLSM). We believe that these experiments demonstrate that we have achieved real time molecular recognition of these small molecules in the vapor phase