Using biomedical sensor - reflectometry interference spectroscopy for evaluation of biocompatibility of biomaterials

Using a biomedical sensor setup RIfS we have investigated the kinetic behavior of bovine serum albumin (BSA), human fibrinogen (FIb) and human IgG adsorbed onto surfaces of hydroxyapatite (HA) and polyurethane (PU) H50-50. According to the operation principle of RIfS and the molecular dimensions of three kinds of proteins, a formula to calculate the adsorbed layers of proteins onto the surface of HA and PU H50-50 has been introduced. The results show that the adsorbed layers of three kinds of proteins on the surface of HA are 1.0751 for IgG 0.9684 for BSA, 0.7464 for FIb and that of PU H50-50 are: 0.8199 for IgG 0.7964 for BSA, 0.6120 for FIb. It is shown that RIfS can perform a kinetic, real time and in situ analysis of plasma proteins adsorbed on a surface of biomaterials. From this study the potential application of RIfS as a new analytical tool in the evaluation of the biocompatibility of biomaterials was confirmed and the experiences of preparing suitable nanograde film from inorganic and organic biomaterials for a RIfS experiment were accumulated.