Title :
Soft lithography for oligonucleotide arrays fabrication
Author :
Xiao, P.F. ; He, N.Y. ; He, Q.G. ; Liu, Z.C. ; Lu, Z.H.
Author_Institution :
Key Lab. of Minist. of Educ. for Molecular & Biomolecular Electron., Southeast Univ., Nanjing, China
Abstract :
A method for fabricating high-density oligonucleotide arrays directed by a set of molecular stamps is reported in this paper. In this method, based on the standard phosphoramidites chemistry protocol, the coupling was conducted under soft lithography. A 20-mer DNA microarray with 10,000 probes has been successfully fabricated, which shows the advantages of accurate, reliable operation and low-cost production of high-density DNA chips.
Keywords :
DNA; arrays; biochemistry; biomolecular electronics; lithography; molecular biophysics; probes; 20-mer DNA microarray; accurate reliable operation; high-density DNA chip production; low-cost production; molecular stamps set; oligonucleotide arrays fabrication; soft lithography; standard phosphoramidites chemistry protocol; Bioinformatics; Chemistry; Chip scale packaging; DNA; Fabrication; Genomics; Glass; Probes; Soft lithography; Spatial resolution;
Conference_Titel :
Engineering in Medicine and Biology Society, 2001. Proceedings of the 23rd Annual International Conference of the IEEE
Print_ISBN :
0-7803-7211-5
DOI :
10.1109/IEMBS.2001.1017458
