DocumentCode :
382293
Title :
Image formation model of a 3D translucent object observed in light microscopy
Author :
Dey, Nicolas ; Boucher, Alain ; Thonnat, Monique
Author_Institution :
INRIA, France
Volume :
2
fYear :
2002
fDate :
2002
Abstract :
We propose a complete model for the image formation of a 3D microscopic translucent object. This model is essentially composed of 3 steps. The first step is the modeling of an object. We define a 3D distribution of complex indexes of refraction. The second step is based on ray tracing and simulates the light propagation inside the specimen. We compute an light intensity volume. Finally, we simulate a light microscope as a blurring model. It forms the 2D final image. This model of blur approximates the real optical transfer function with 5 physical parameters describing the optical system. We show results of our complete model. The computed images are compared to real images of a microscopic glass sphere.
Keywords :
image processing; optical microscopy; optical transfer function; ray tracing; refractive index; 2D image; 3D distribution; 3D translucent object; blurring model; complex indexes; complex indexes of refraction; image formation model; light intensity volume; light microscopy; light propagation simulation; microscopic glass sphere; object modeling; optical transfer function; physical parameters; ray tracing; Absorption; Computational modeling; Fluorescence; Focusing; Image sequences; Light sources; Optical microscopy; Optical refraction; Ray tracing; Shape;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Image Processing. 2002. Proceedings. 2002 International Conference on
ISSN :
1522-4880
Print_ISBN :
0-7803-7622-6
Type :
conf
DOI :
10.1109/ICIP.2002.1039989
Filename :
1039989
Link To Document :
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