Usage of multi anode PMT on the multi-photon fluorescence spectroscopy and video rate microscopy

Author

Hean Kim, Ki ; Buehler, Christof ; Bahlmann, Karsten ; So, Peter T.C.

Volume

2

fYear

2002

fDate

2002

Abstract

Summary form only given. We developed multi-photon fluorescence spectroscopy based on a 16×1 multianode photo multiplier tube and we continue to develop video rate microscopy based on an 8×8 multianode PMT. The spectroscopy consists of grating, 16×1 multianode PMT and a 16 channel photon counting card. It can take spectrum of pixels in addition to acquiring images. Multiple color fluorescence spheres, cells stained with multiple color fluorescence dyes and tissues were examined with the system. Video rate multi-photon microscopy is constructed based on an 8×8 micro lenslet array, an 8×8 multianode PMT and 64 photon counting card for deep tissue imaging which is difficult due to scattering of tissue samples.

Keywords

biological techniques; biological tissues; biomedical optical imaging; cellular biophysics; fluorescence spectroscopy; microlenses; multiphoton spectroscopy; optical microscopy; photomultipliers; photon counting; 16 channel photon counting card; deep tissue imaging; grating; micro lenslet array; multi anode PMT; multi-photon fluorescence spectroscopy; multianode photomultiplier tube; multiple color fluorescence dye stained cells; multiple color fluorescence spheres; pixels; tissue sample scattering; tissues; video rate microscopy; Anodes; Electromagnetic scattering; Fluorescence; Gratings; Microscopy; Particle scattering; Pixel; Spectroscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint

ISSN

1094-687X

Print_ISBN

0-7803-7612-9

Type

conf

DOI

10.1109/IEMBS.2002.1106350

Filename

1106350