Title :
Usage of multi anode PMT on the multi-photon fluorescence spectroscopy and video rate microscopy
Author :
Hean Kim, Ki ; Buehler, Christof ; Bahlmann, Karsten ; So, Peter T.C.
Abstract :
Summary form only given. We developed multi-photon fluorescence spectroscopy based on a 16×1 multianode photo multiplier tube and we continue to develop video rate microscopy based on an 8×8 multianode PMT. The spectroscopy consists of grating, 16×1 multianode PMT and a 16 channel photon counting card. It can take spectrum of pixels in addition to acquiring images. Multiple color fluorescence spheres, cells stained with multiple color fluorescence dyes and tissues were examined with the system. Video rate multi-photon microscopy is constructed based on an 8×8 micro lenslet array, an 8×8 multianode PMT and 64 photon counting card for deep tissue imaging which is difficult due to scattering of tissue samples.
Keywords :
biological techniques; biological tissues; biomedical optical imaging; cellular biophysics; fluorescence spectroscopy; microlenses; multiphoton spectroscopy; optical microscopy; photomultipliers; photon counting; 16 channel photon counting card; deep tissue imaging; grating; micro lenslet array; multi anode PMT; multi-photon fluorescence spectroscopy; multianode photomultiplier tube; multiple color fluorescence dye stained cells; multiple color fluorescence spheres; pixels; tissue sample scattering; tissues; video rate microscopy; Anodes; Electromagnetic scattering; Fluorescence; Gratings; Microscopy; Particle scattering; Pixel; Spectroscopy;
Conference_Titel :
Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint
Print_ISBN :
0-7803-7612-9
DOI :
10.1109/IEMBS.2002.1106350
