Title :
Flow induced platelet activation in mechanical heart valves - in vitro studies
Author :
Yin, Wei ; Bluestein, Danny ; Jesty, Jolyon ; Perrotta, Peter
Author_Institution :
Biomed. Eng. Dept., State Univ. of New York, Stony Brook, NY, USA
Abstract :
Thromboembolism is a common complication of mechanical heart valves (MHV). The objective of this study was to elucidate mechanisms of thromboembolism in flow past MHV in vitro. Gel-filtered platelets were recirculated through the MHV mounted in a model of left ventricular assist device (LVAD) and their activation was measured by using an innovative platelet activity state (PAS) assay. The motion of one MHV in LVAD was restricted by suture, and LVAD with free MHV was considered as control. The platelet activation increased as a function of the recirculation time past the valve, and the restricted valve had a higher activation rate as compared to controls. Flow cytometry was conducted to compare the results obtained from PAS assay.
Keywords :
artificial organs; cardiology; haemodynamics; physiological models; prosthetics; valves; activation rate; flow cytometry; flow induced platelet activation; free MHV; gel-filtered platelets; in vitro studies; left ventricular assist device; mechanical heart valves; platelet activity state assay; recirculation time; restricted valve; suture; thromboembolism; Biomedical engineering; Biomedical measurements; Filtration; Heart valves; Hemorrhaging; In vitro; Motion control; Pathology; Plasma measurements; Prosthetics;
Conference_Titel :
Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint
Print_ISBN :
0-7803-7612-9
DOI :
10.1109/IEMBS.2002.1106557
