Title :
Towards portable flow cytometry: study on the use of air-sheath-based volume-efficient two-phase microfluidic systems
Author :
Huh, Dongeun ; Tkaczyk, Alan H. ; Wei, Hsien-Hung ; Grotberg, James B. ; Takayama, Shuichi
Author_Institution :
Dept. of Biomed. Eng., Michigan Univ., Ann Arbor, MI, USA
Abstract :
Reports experimental studies on air-liquid two-phase stratified flows in polymeric microfluidic channels for volume-efficient and cost-effective flow cytometry. Different flow behaviors of liquid core streams sheathed by air flows were investigated by varying surface chemistry and geometry of poly(dimethylsiloxane) (PDMS) channels. Fluorescent beads and myoblast cells carried by stable liquid flows were analyzed by a digital photo-multiplier tube (PMT)-based optical detection system to analyze the number of particles in a sample.
Keywords :
biological techniques; cellular biophysics; fluorescence; microfluidics; spectrochemical analysis; stratified flow; surface chemistry; two-phase flow; PDMS channels; air flows; air-liquid two-phase stratified flows; air-sheath-based volume-efficient two-phase microfluidic systems; cost-effective flow cytometry; digital photomultiplier tube-based optical detection system; epifluorescence microscope; fluorescent beads; geometry; liquid core streams; myoblast cells; number of particles; poly(dimethylsiloxane); polymeric microfluidic channels; portable flow cytometry; stable liquid flows; surface chemistry; volume-efficient flow cytometry; Aerodynamics; Biomedical engineering; Biomedical optical imaging; Fluid flow; Fluorescence; Image motion analysis; Microfluidics; Optical detectors; Optical microscopy; Optical pumping;
Conference_Titel :
Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint
Print_ISBN :
0-7803-7612-9
DOI :
10.1109/IEMBS.2002.1106579
