Title :
Efficient Acquisition and Learning of Fluorescence Microscope Data Models
Author :
Charles Jackson;Robert F. Murphy;Jelena Kovacevic
Author_Institution :
Dept. of Biomedical Eng. and Center for Bioimage Informatics, Carnegie Mellon University, Pittsburgh, PA, USA
Abstract :
We present a method for efficient acquisition of fluorescence microscope datasets, to allow for higher spatial and temporal resolution, and with less damage from photobleaching. Our proposal is to restrict acquisition to regions where we expect to find an object. Given that the objects are continuously moving, we must have an accurate model to describe objects´ motion to predict their future locations. We outline a system for learning and applying this motion model, provide details from some simple simulations, and summarize results from more complex applications.
Keywords :
"Fluorescence","Microscopy","Data models","State-space methods","Photobleaching","Machine learning","Tracking","Equations","Spatial resolution","Biological system modeling"
Conference_Titel :
Image Processing, 2007. ICIP 2007. IEEE International Conference on
Print_ISBN :
978-1-4244-1436-9
Electronic_ISBN :
2381-8549
DOI :
10.1109/ICIP.2007.4379567