DocumentCode :
386341
Title :
The contribution of adhesion forces to the leukocyte response to shear stress
Author :
Coughlin, M.F. ; Schmid-Schonbein, G.W.
Author_Institution :
Dept. of Bioeng., Univ. of California, San Diego, CA, USA
Volume :
1
fYear :
2002
fDate :
2002
Firstpage :
308
Abstract :
Leukocyte emigration at inflammatory sites requires rolling along microvessels, firm adhesion, cell spreading and active migration. While these activities require leukocytes to shift from a passive to an active state, the mechanisms underlying these phenomena are incompletely understood. The leukocyte response to fluid shear stress may play a regulatory role. We previously showed that glass-adherent and initially passive leukocytes exposed to a controlled fluid shear stress project pseudopodia, extend lamellipodia and extensively spread in an amplitude and duration dependent manner. The aim of the current investigation was to elucidate mechanisms underlying the transformation of adherent leukocytes from a round to spread configuration by fluid shear stress. Several approaches were used to manipulate membrane adhesive and repulsive forces in human leukocytes before exposure to fluid shear stress of ∼2 dyn/cm2. Cell geometric parameters from each of the interventions were compared to control values. Manipulating adhesion forces both enhanced and diminished the cell spreading response to fluid shear stress.
Keywords :
adhesion; biomembrane transport; haemorheology; shear flow; wetting; adherent leukocytes; adhesion forces; biomechanics; biomembrane transport; blood; cell geometric parameters; fluid shear stress; inflammatory sites; lamellipodia; leukocyte emigration; leukocyte response; mechanotransduction; membrane forces; microvessels; pseudopodia; repulsive forces; round configuration; shear flow; spread configuration; Adhesives; Biomedical engineering; Biomembranes; Cells (biology); Ethanol; Glass; Humans; Plasmas; Stress control; White blood cells;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint
ISSN :
1094-687X
Print_ISBN :
0-7803-7612-9
Type :
conf
DOI :
10.1109/IEMBS.2002.1134507
Filename :
1134507
Link To Document :
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