Title :
Rapid, multipoint molecular spectroscopic measurements of shear effects on endothelial cell membranes
Author :
Butler, Peter J. ; Bowen, Benjamin P. ; Woodbury, Neal
Author_Institution :
Dept. of Bioeng., Pennsylvania State Univ., University Park, PA, USA
Abstract :
Endothelial cells (ECs) form the inner lining; of the blood vasculature and are therefore exposed to shear stress (tau), the tangential component of hemodynamic forces. Shear stress exacts potent changes in EC biology, possibly via EC-membrane perturbations, with consequent influences on vascular health. We describe a time-correlated single photon counting method for measuring rapid and spatially heterogeneous changes in fluorescence lifetimes and polarizations of EC membranes exposed to shear stress. The role in mechanotransduction by ECs of shear-induced changes in membrane lipid dynamics is discussed.
Keywords :
biomembrane transport; fluorescence; fluorescence spectroscopy; haemorheology; lipid bilayers; molecular biophysics; optical microscopy; shear flow; blood vasculature; endothelial cell membranes; fluorescence lifetimes; hemodynamic forces; mechanotransduction; membrane lipid dynamics; membrane perturbations; molecular spectroscopic measurements; polarization; shear effects; time-correlated single photon counting method; vascular health; Biomedical engineering; Biomembranes; Cells (biology); Fluorescence; Lipidomics; Polarization; Position measurement; Spectroscopy;
Conference_Titel :
Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint
Print_ISBN :
0-7803-7612-9
DOI :
10.1109/IEMBS.2002.1134513
