Title :
In situ tensile load modulates inhibition of MMP-1 expression in tendons cells
Author :
Arnoczky, S.P. ; Tian, T. ; Lavagnino, M.
Author_Institution :
Lab. for Comparative Orthopaedic Res., Michigan State Univ., East Lansing, MI, USA
Abstract :
To determine the effect of various degrees of tensile loading on the expression of collagenase (MMP-1) in tendon cells, rat tail tendons were statically stressed at 0.16, 0.75, and 25 MPa for 24 hours. In addition, tendons were cyclically stressed (0.017 Hz) at 6% strain (25 MPa) for 24 hours. Northern blot analysis was used to assay for mRNA expression of MMP-1 in stress-deprived as well as stressed tendons. Stress-deprivation for 24 hours resulted in a marked upregulation in the mRNA expression of MMP-1. Static tensile load was found to significantly (but not completely) inhibit mRNA expression of MMP-1 in a dose dependent manner. Low frequency cyclic stress, however, completely inhibited MMP-1 expression. The results of this study suggest that MMP-1 expression in tendon cells can be regulated by tensile stress, presumably through a mechanotransduction pathway.
Keywords :
biomechanics; cellular biophysics; proteins; 0.017 Hz; 24 h; MMP-1 expression inhibition modulation; Northern blot analysis; cellular biomechanics; collagenase; cyclically stressed tendons; in situ tensile load; mRNA expression inhibition; mechanotransduction pathway; stress-deprived tendons; tendon cells; tensile modulus; Analysis of variance; Capacitive sensors; Frequency; Laboratories; Optical films; Probes; RNA; Tail; Tendons; Tensile stress;
Conference_Titel :
Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint
Print_ISBN :
0-7803-7612-9
DOI :
10.1109/IEMBS.2002.1136878
