DocumentCode :
386453
Title :
New degradable cationic polyesters for nonviral gene delivery
Author :
Fu, Jie ; Krauland, Eric ; Har-el, Yah-el ; Hanes, Justin
Author_Institution :
Dept. of Chem. Eng., Johns Hopkins Univ., Baltimore, MD, USA
Volume :
1
fYear :
2002
fDate :
2002
Firstpage :
551
Abstract :
A new family of biodegradable cationic polyesters consisting of aspartic acid and aliphatic diols of various lengths has been synthesized. Since the polymers formed are strictly alternating, the cationic charge density can be controlled by simply changing the size of the spacer aliphatic monomer. One such polymer, poly(aspartic anhydride-co-ethylene glycol) (PAE), was found to be capable of self-assembly (∼100 nm) into polymeric micelles and complexation with DNA. Kinetic studies reveal PAE initially complexes DNA into sub-100 nm complexes and subsequently releases it after 3-6 days at room temperature. With the ability to degrade and unpack its genetic material, this new family of biodegradable cationic polymers shows promise as versatile gene carriers for in vitro and in vivo applications.
Keywords :
biomedical materials; cellular transport; genetics; patient treatment; polymer solutions; 100 nm; 3 to 6 d; DNA complexation; aliphatic diols; aspartic acid; biodegradable cationic polymers; cationic charge density control; degradable cationic polyesters; genetic material unpacking; in vitro applications; in vivo applications; nonviral gene delivery; poly(aspartic anhydride-co-ethylene glycol); polymeric micelles; room temperature; self-assembly; strictly alternating polymers; versatile gene carriers; Amino acids; Biodegradable materials; DNA; Genetics; Kinetic theory; Polymers; Self-assembly; Size control; Temperature; Thermal degradation;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint
ISSN :
1094-687X
Print_ISBN :
0-7803-7612-9
Type :
conf
DOI :
10.1109/IEMBS.2002.1136945
Filename :
1136945
Link To Document :
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