DocumentCode :
386454
Title :
Characterization of an electrospun poly(lactide-co-glycolide) and block copolymer-based, nanostructured matrix for DNA delivery
Author :
Luu, Y.K. ; Kim, K. ; Hsiao, B.S. ; Chu, B. ; Hadjiargyrou, M.
Author_Institution :
Dept. of Biomed. Eng., State Univ. of New York, Stony Brook, NY, USA
Volume :
1
fYear :
2002
fDate :
2002
Firstpage :
553
Abstract :
While gene delivery represents a promising means of engineering biological tissue, many obstacles need to be overcome before the promise of the technology can be fulfilled. Chief among these obstacles is the development of non-viral gene delivery. The objectives of the present work were to fabricate and characterize the release properties of an electrospun DNA/polymer nanostructured membrane. Release of DNA from 1.5 × 1 cm2 sections of membrane was assayed in phosphate buffered saline (PBS) and tris-EDTA solution (TE). The release profile of DNA from the membranes exhibited sustained release over an eight-day study period, with maximum release occurring at ∼ 2 hours. Cumulative release profiles were similar for release in TE and PBS, with amounts released being approximately 20-30% of the initially loaded DNA. One of the critical questions addressed by this study was the assessment of DNA integrity post-processing as determined by gel electrophoresis. Results indicate that DNA released from an electrospun membrane is indeed intact, and should be capable of cellular transfection. Future work on the project will be to increase and optimize the release of DNA from the membranes in preparation for in vitro and in vivo studies assessing transfection efficiency of released plasmid.
Keywords :
DNA; biomedical materials; biomembrane transport; genetics; nanostructured materials; patient treatment; polymer blends; 2 hr; 8 d; DNA delivery; DNA integrity assessment; DNA release profile; block copolymer-based nanostructured matrix; cellular transfection; cumulative release profiles; electrospun poly(lactide-co-glycolide); in vitro studies; in vivo studies; nonviral gene delivery; phosphate buffered saline; released plasmid; tissue engineering; transfection efficiency; tris-EDTA solution; Biomedical engineering; Biomembranes; DNA; In vivo; Materials science and technology; Nanobioscience; Polymers; Regeneration engineering; Tellurium; Tissue engineering;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint
ISSN :
1094-687X
Print_ISBN :
0-7803-7612-9
Type :
conf
DOI :
10.1109/IEMBS.2002.1136946
Filename :
1136946
Link To Document :
بازگشت