DocumentCode :
386517
Title :
Differential effects of lactacystin on IL-1 beta and TNF-alpha induced adhesion molecule expression and myeloid cell adhesion to endothelial cells
Author :
Dagia, Nilesh M. ; Goetz, Douglas J.
Author_Institution :
Dept. of Chem. Eng., Ohio Univ., Athens, OH, USA
Volume :
1
fYear :
2002
fDate :
2002
Firstpage :
680
Abstract :
Proteasome inhibitors (e.g. lactacystin) have been shown to decrease cytokine-induced up-regulation of endothelial cell adhesion molecules and consequent leukocyte adhesion. The majority of these studies have focused on the effects of proteasome inhibitors on endothelial cells exposed to a single cytokine (TNF alpha) for 4 hrs. However, in the in vivo setting, multiple cytokines (e.g. IL-1 beta and TNF alpha) may be present and their levels may be increased for extended periods off time. In addition, the effects of IL-1 beta (IL-1) and TNF alpha (TNF) on endothelial cells are not identical. Thus, we studied the effect of lactacystin on IL-1 and TNF induced adhesion molecule expression and myeloid cell (HL-60 cells) adhesion to human umbilical vein endothelial cells (HUVEC). We found that (a) lactacystin inhibits 4 hr. IL-1 or TNF induced expression of E-selectin, ICAM-1, VCAM-1 and HL-60 cell adhesion to HUVEC, (b) lactacystin differentially inhibits transient adhesive contacts of HL-60 cells to 4 hr. IL-1 and TNF activated HUVEC, (c) lactacystin inhibits 4 hr. TNF induced expression of VCAM-1, E-selectin and HL-60 cell adhesion to IL-1 sefractory HUVEC and (d) lactacystin inhibits HL-60 cell adhesion to 24 hr. TNF activated HUVEC.
Keywords :
adhesion; biomechanics; cellular biophysics; molecular biophysics; proteins; 24 hr; 4 hr; HL-60 cells; TNF-alpha induced adhesion molecule expression; differential effects; endothelial cells; human umbilical vein endothelial cells; in vivo setting; lactacystin on IL-1 beta; leukocyte; multiple cytokines; myeloid cell adhesion; proteasome inhibitors; Adhesives; Chemical engineering; Humans; In vivo; Inhibitors; Probes; Proteins; Protocols; Veins; White blood cells;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint
ISSN :
1094-687X
Print_ISBN :
0-7803-7612-9
Type :
conf
DOI :
10.1109/IEMBS.2002.1137014
Filename :
1137014
Link To Document :
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