Cytocidal and inhibitory effect of energy-controllable pulse on ovarian cancer cell line SKOV3

Author

Sun, C.X. ; Xiong, L. ; Yao, C.G. ; Mi, Y. ; Hu, Y. ; Hu, L.N.

Author_Institution

Key Lab of High Voltage Eng. & Electr. New Technol., Minist. of Educ., Chongqing, China

Volume

1

fYear

2002

fDate

2002

Firstpage

703

Abstract

Our objective was to study the cytocidal and inhibitory effect of energy controllable pulse on ovarian cancer cell line SKOV3. An ovarian cancer cell suspension was treated by electric pulses with different parameters. Inhibitory rate (IR) was assayed by modified colorimetric MTT methods. Growth curves of two test groups and one control group were also measured, and morphological changes were observed under transmission electron microscopy (TEM). Treated SKOV3 cell proliferated more slowly. IR promoted with the enhancement of each pulse parameter. The morphological study showed that ultrastructural changes occurred obviously. Swollen mitochondria, fractured ridges, cytoplasmic vacuoles and membrane holes appeared in most of processed cell, and part of bilayer membrane was ruptured. In conclusion, irreversible electric breakdown occurred in part of treated cells, and an electric pulse with proper intensity could kill cancer cells and inhibit their recovery and growth.

Keywords

bioelectric phenomena; biological effects of fields; biomembranes; cancer; cellular effects of radiation; electric field effects; gynaecology; bilayer membrane rupture; cytoplasmic vacuoles; energy-controllable pulse; fractured ridges; inhibitory rate; irreversible breakdown; membrane holes; ovarian cancer cell suspension; proliferation inhibition; swollen mitochondria; Biomedical engineering; Biomedical measurements; Biomembranes; Cancer; Electric breakdown; Frequency; Medical treatment; Power engineering and energy; Testing; Voltage;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint

ISSN

1094-687X

Print_ISBN

0-7803-7612-9

Type

conf

DOI

10.1109/IEMBS.2002.1137026

Filename

1137026