DocumentCode :
386582
Title :
Tissue engineering of intact arterial segments by directed remodeling
Author :
Nichol, J.W. ; Clerin, V. ; Gooch, K.J.
Author_Institution :
Dept. of Bioeng., Pennsylvania Univ., Philadelphia, PA, USA
Volume :
1
fYear :
2002
fDate :
2002
Firstpage :
819
Abstract :
Traditional approaches of generating tissue-engineered arteries in vitro rely on expansion of cells in culture to seed onto appropriate scaffolds. We propose that small autologous arteries, not the cells derived from them, may be an attractive starting point for engineered arteries. This approach capitalizes on the ability of intact arteries to grow and remodel in response to chronic changes in their mechanical environment. Carotid arteries from juvenile (∼30-kg) pigs were stretched longitudinally in an ex vivo perfusion system over 9 days. This resulted in a ∼40% increase in artery length at physiological longitudinal stress and a 20 ± 3% increase when unstressed. In contrast, control arteries perfused for 9 days at their physiological loaded length saw no unstressed length increase. Control and elongated arteries displayed native appearance (macroscopic and histological), excellent viability (cellularity and mitochondrial activity), normal vasoactivity, and similar mechanical properties (ultimate stress and ultimate strain) as compared to freshly harvested arteries. Growth, as opposed to just redistribution of existing mass, contributed to elongation as evidenced by an increase in artery weight. Elongated arteries also maintained similar wall thickness as fresh arteries, whereas control arteries decreased significantly.
Keywords :
biomechanics; blood vessels; cellular biophysics; patient treatment; 9 d; artery weight; control arteries; directed remodeling; elongated arteries; ex vivo perfusion system; freshly harvested arteries; intact arterial segments; juvenile pigs; mechanical effects; mechanical properties; normal vasoactivity; physiological longitudinal stress; tissue engineering; ultimate strain; ultimate stress; vascular biology; vascular prostheses; wall thickness maintenance; Capacitive sensors; Carotid arteries; Cells (biology); In vitro; Mechanical factors; Prosthetics; Steel; Stress; Surgery; Tissue engineering;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint
ISSN :
1094-687X
Print_ISBN :
0-7803-7612-9
Type :
conf
DOI :
10.1109/IEMBS.2002.1137091
Filename :
1137091
Link To Document :
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