Development of a YIGSR peptide-modified polyurethaneurea to enhance endothelialization

We have developed a peptide-modified bioactive polyurethaneurea to enhance vascular graft endothelialization. YIGSR peptides were incorporated into the polymer backbone, and successful incorporation of the peptides was confirmed by NMR, contact angle measurement, and ESCA. Uniform distribution of peptides on the surface was observed using a fluorescent probe capable of reacting with tyrosine residues on the peptides. Hard segment domains were observed to be arranged parallel to the plane of the surface using AFM. Endothelial cell adhesion, spreading, proliferation, migration, and extracellular matrix production were improved on bioactive polyurethaneurea (PUUYIGSR) compared to control (PUUPPD). Additionally, competitive inhibition of endothelial cell attachment and spreading was found when cells were incubated in the presence of soluble YIGSR peptides.