Fluid flow increases mineralized matrix deposition in three-dimensional perfusion culture of marrow stromal osteoblasts in a dose-dependent manner

The aim of the study is to investigate the effect of flow perfusion in three-dimensional titanium meshes seeded with rat marrow stromal cells (MSCs), on the ability of these cells to proliferate, differentiate towards the osteoblastic lineage, and generate mineralized extracellular matrix. Flow perfusion allows the continuous infusion of cell culture media throughout the porous network of the scaffold. It also permits the mechanical stimulation of the seeded cells by exposing them to shear forces.