Title :
Non-invasiveness, high cell viability, and high penetration of multi-harmonic generation microscopy
Author :
Sun, Chi-Kuang ; Chu, Shi-Wei ; Chen, Szu-Yu ; Tsai, Tsung-Han ; Liu, Tzu-Ming ; Lin, Chung-Yung ; Tsai, Huai-Jen
Author_Institution :
Graduate Inst. of Electro-Opt. Eng., Nat. Taiwan Univ., Taipei, Taiwan
Abstract :
This paper studies live zebrafish embryos as examples in discussing penetration, cell viability, and non-invasiveness issues of harmonic generation microscopy. No optical damage can be observed during the whole proliferation process even with 100-mW incident average power on the embryo after long-term (>12 hrs) observations.
Keywords :
bio-optics; biological techniques; cellular biophysics; laser beam applications; optical harmonic generation; optical microscopy; 100 mW; cell viability; live zebrafish embryos; multiharmonic generation microscopy; noninvasive technique; penetration; proliferation process; Biomedical optical imaging; Embryo; Fiber nonlinear optics; Fluorescence; Laser excitation; Nonlinear optics; Optical harmonic generation; Optical microscopy; Optical scattering; Ultrafast optics;
Conference_Titel :
Lasers and Electro-Optics Society, 2003. LEOS 2003. The 16th Annual Meeting of the IEEE
Print_ISBN :
0-7803-7888-1
DOI :
10.1109/LEOS.2003.1251725
