DocumentCode
399933
Title
Non-invasiveness, high cell viability, and high penetration of multi-harmonic generation microscopy
Author
Sun, Chi-Kuang ; Chu, Shi-Wei ; Chen, Szu-Yu ; Tsai, Tsung-Han ; Liu, Tzu-Ming ; Lin, Chung-Yung ; Tsai, Huai-Jen
Author_Institution
Graduate Inst. of Electro-Opt. Eng., Nat. Taiwan Univ., Taipei, Taiwan
Volume
1
fYear
2003
fDate
27-28 Oct. 2003
Firstpage
239
Abstract
This paper studies live zebrafish embryos as examples in discussing penetration, cell viability, and non-invasiveness issues of harmonic generation microscopy. No optical damage can be observed during the whole proliferation process even with 100-mW incident average power on the embryo after long-term (>12 hrs) observations.
Keywords
bio-optics; biological techniques; cellular biophysics; laser beam applications; optical harmonic generation; optical microscopy; 100 mW; cell viability; live zebrafish embryos; multiharmonic generation microscopy; noninvasive technique; penetration; proliferation process; Biomedical optical imaging; Embryo; Fiber nonlinear optics; Fluorescence; Laser excitation; Nonlinear optics; Optical harmonic generation; Optical microscopy; Optical scattering; Ultrafast optics;
fLanguage
English
Publisher
ieee
Conference_Titel
Lasers and Electro-Optics Society, 2003. LEOS 2003. The 16th Annual Meeting of the IEEE
ISSN
1092-8081
Print_ISBN
0-7803-7888-1
Type
conf
DOI
10.1109/LEOS.2003.1251725
Filename
1251725
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