Title :
A variable-focus lens with 1 kHz bandwidth applied to axial-scan of a confocal scanning microscope
Author :
Oku, Hironiasa ; Ishikawa, Masatoshi
Author_Institution :
Intelligent Cooperation & Control, Japan Sci. & Technol. Corp., Tokyo, Japan
Abstract :
This paper proposes a new structure of variable-focus lens aimed at 1 kHz bandwidth. Two kinds of prototypes are developed to validate the structure. The 1 kHz bandwidth is confirmed by the frequency response measurements. One prototype is applied to the axial high-speed scan of a confocal laser scanning microscope. Depth information measurements of a microscopic object in 1 ms and automated focusing to keep the focus on a randomly moving object are successfully achieved.
Keywords :
biological techniques; high-speed optical techniques; laser beam applications; lenses; near-field scanning optical microscopy; optical focusing; optical variables measurement; 1 ms; automated focusing; axial-scan; confocal laser scanning microscope; confocal scanning microscope; depth information measurements; frequency response measurements; high-speed scan; microscopic object; randomly moving object; variable-focus lens; Actuators; Bandwidth; Focusing; Frequency measurement; Frequency response; Glass; Lenses; Optical design; Optical microscopy; Prototypes;
Conference_Titel :
Lasers and Electro-Optics Society, 2003. LEOS 2003. The 16th Annual Meeting of the IEEE
Print_ISBN :
0-7803-7888-1
DOI :
10.1109/LEOS.2003.1251762