Reproducibility of measurement of organophosphorous pesticides toxicity

Fast detection of pesticide toxicity in the field conditions is very important in many aspects (pesticide storage, price of one test etc.). The measurement uses principle of an artificial synapse (AS) and inhibition of an enzyme Acetylcholinesterase (AChE). The AS is a very effective detector of the pesticide detector. This information is very different from classical analytic method data. The enzyme is sensitive to many external influences and its function is significantly influenced by immobilization process. Therefore properties of the sensor have to be measured without AChE at first. This process is called input control of the sensor. The reproducibility of electrochemical detector was studied first using hydrogen peroxide and second ferri-ferrokyanide redox couple. Longterm stability and reproducibility of preparation of enzymatic layer were studied. Cell data was evaluated statistically. This article describes measurement on AS and determination properties of electrochemical sensor before immobilization of enzymes by hydrogen peroxide.