Title :
Optimization of targeted therapies to inhibit smooth muscle cell invasion in vitro
Author :
Kennedy, C.E. ; Massia, S.P.
Author_Institution :
Harrington Dept. of Bioeng., Arizona State Univ., Tempe, AZ, USA
Abstract :
Vascular smooth muscle cell (VSMC) proliferation and migration has been correlated with intimal hyperplasia (IH) after vascular interventions such as angioplasty, stenting, and vascular graft surgery. Therefore, therapies targeting inhibition of VSMC migration may lead to higher patency rates in vascular grafts and reduced IH in other vascular interventions. This study tested three targeted therapies and their combinations: hyperfunctional αvβ3integrin expression, cyclic-RGD release and tissue inhibitor of metalloprotease (TIMP)-1 release. It was found that a combination of 1.0 mM cyclic-RGD and 10 ng/mL TIMP-1 maximally inhibited smooth muscle cell invasion over individual or other combinations of treatments over 72 hours.
Keywords :
biological tissues; biomedical engineering; cardiovascular system; cell motility; diseases; muscle; proteins; surgery; 72 hours; angioplasty stenting; cell migration; cyclic-RGD release; hyperfunctional αvβ3integrin expression; intimal hyperplasia; optimization; targeted therapy; tissue inhibitor of metalloprotease (TIMP)-1 release; vascular graft surgery; vascular interventions; vascular smooth muscle cell proliferation; Angioplasty; Arteries; Biomembranes; Fluorescence; In vitro; Medical treatment; Muscles; Protocols; Sliding mode control; Surgery;
Conference_Titel :
Engineering in Medicine and Biology Society, 2003. Proceedings of the 25th Annual International Conference of the IEEE
Print_ISBN :
0-7803-7789-3
DOI :
10.1109/IEMBS.2003.1279470
