Dendranthema morifolium attenuated the reduction of contraction of isolated heart and cardiomyocytes induced by ischemia/reperfusion and anoxia/reoxygenation

The aim of the present study is to investigate the effect of Dendranthema morifolium (DrVI) on isolated rat heart and ventricular myocytes during ischemia/anoxia and reperfusion/reoxygenation. The Langendorff perfused rat hearts were used to measure intraventricular pressure and coronary flow. The cell contraction in enzymatically isolated ventricular myocytes was determined by a video tracking system. DM significantly attenuated the inhibitory effects induced by ischemia/reperfusion on left ventricular developed pressure (LVDP), ±dP/dtmax, coronary flow and LVDP×HR. DM attenuated the inhibitory effects of anoxia and reoxygenation on cell contraction in isolated ventricular myocytes. The results suggest that DM processes cardioprotective effect during ischemia/anoxia and reperfusion/reoxygenation in the isolated rat heart and the ventricular myocytes.