DocumentCode
411701
Title
All optical histology of brain tissue: serial ablation and multiphoton imaging with femtosecond laser pulses
Author
Tsai, Philbert S. ; Friedman, Beth ; Lev-Ram, Varda ; Xiong, Qing ; Tsien, Roger Y. ; Kleinfeld, David ; Ifarraguerri, Agustin I. ; Thompson, Beverly D. ; Squier, Jeff A.
Author_Institution
Dept. of Phys. & Pharmacology, California Univ., San Diego, La Jolla, CA, USA
fYear
2003
fDate
6-6 June 2003
Abstract
We demonstrate the first use of femtosecond laser pulses for serial histology. Successive iterations of multiphoton imaging and ablation provide diffraction-limited volumetric data that is used to reconstruct the architectonics of labeled cells or microvasculature.
Keywords
biological effects of laser radiation; biomedical optical imaging; blood vessels; brain; cellular biophysics; high-speed optical techniques; iterative methods; laser ablation; multiphoton processes; brain tissue; diffraction-limited volumetric data; femtosecond laser pulse; labeled cells; microvasculature; multiphoton imaging; optical histology; serial ablation; successive iteration; Brain; Fluorescence; Laser ablation; Optical imaging; Optical pulses; Optical scattering; Proteins; Rough surfaces; Surface roughness; Ultrafast optics;
fLanguage
English
Publisher
ieee
Conference_Titel
Lasers and Electro-Optics, 2003. CLEO '03. Conference on
Conference_Location
Baltimore, MD, USA
Print_ISBN
1-55752-748-2
Type
conf
Filename
1297781
Link To Document