Title :
A rapid method for detection of recombinant proteins in single Pichia yeast cell with optical Raman-tweezers spectroscopy
Author :
Xie, Changan ; Zhu, Yong ; Li, Yong-qing
Author_Institution :
Dept. of Phys., East Carolina Univ., Greenville, NC, USA
Abstract :
Optical Raman-tweezers spectroscopy was used to detect recombinant proteins (somatolactin) produced in single Pichia yeast cells, in which the gene of zebrafish somatolactin /spl beta/ has been inserted and expressed in the transgenic cells.
Keywords :
Raman spectroscopy; biological techniques; cellular biophysics; genetics; microorganisms; molecular biophysics; proteins; radiation pressure; optical Raman-tweezers spectroscopy; recombinant proteins; single Pichia yeast cell; transgenic cells; zebrafish somatolactin /spl beta/;
Conference_Titel :
Lasers and Electro-Optics, 2004. (CLEO). Conference on
Conference_Location :
San Francisco, CA
Print_ISBN :
1-55752-777-6
