Title :
Identification of a switching model of calcium cycling in isolated rat hearts
Author :
Den Buijs, J. Op ; Juloski, A.L. ; Ivanics, T. ; Ligeti, L. ; van der Vusse, G.J. ; van Riel, N.A.W.
Author_Institution :
Dept. of Electr. Eng., Eindhoven Univ. of Technol., Netherlands
Abstract :
So far, the processes involved in regulation of intracellular calcium (Cai2+) in cardiomyocytes have been mainly studied through biochemical and isolated cell analysis. Here, we present a novel technique to model and identify cardiac Cai2+-cycling under physiologically relevant conditions in the intact beating heart. Cai2+ was measured using fluorescence techniques in ex vivo perfused rat hearts. For analysis, we developed a parametric mathematical model, switching between active and inactive calcium release. The kinetic parameters of the two submodes of the model were computed using a recently developed technique from hybrid system identification. Application of the method to control and isoproterenol-stimulated hearts resulted in parameter values within a physiologically reliable range.
Keywords :
biochemistry; biorheology; calcium; cardiology; cellular biophysics; fluorescence; muscle; physiological models; Ca; biochemistry; calcium cycling switching model; cardiac Ca/sub i//sup 2+/-cycling; cardiomyocytes; fluorescence technique; hybrid system identification; intracellular calcium; isolated cell analysis; isolated rat heart; parametric mathematical model; Biomembranes; Calcium; Heart; Kinetic theory; Mercury (metals); Performance evaluation; Proteins; Rats; Steady-state; Strontium; Calcium; Hybrid systems; Identification; Mathematical model; Perfused Heart;
Conference_Titel :
Engineering in Medicine and Biology Society, 2004. IEMBS '04. 26th Annual International Conference of the IEEE
Conference_Location :
San Francisco, CA
Print_ISBN :
0-7803-8439-3
DOI :
10.1109/IEMBS.2004.1403289