Title :
Real-time estimation of oxygen concentration in micro-hemo-vessels
Author :
De Francisci, M. ; Bucolo, M. ; Intaglietta, M. ; Arena, P. ; Fortuna, L.
Author_Institution :
Dipt. di Ingegneria Elettrica, Elettronica e dei Sistemi, Univ. degli Studi di Catania, Italy
Abstract :
A real-time measurement system for non-invasive evaluation of oxygen concentration (PO2) at the microcirculation level is developed. The system has been designed by exploiting the phenomenon of fluorescence quenching. The skin of an anaesthetized hamster, injected with porphyrin, is lighted with pulses; the fluorophore reacts with the oxygen in the blood, producing a fluorescence signal, and the value of the fluorescence lifetime is related to the oxygen concentration. This microcirculation-based instrumentation consists of an electro-optical system, a control circuit and signal processing procedure. The system allows the measurement of PO2 in the range of 0-700 (mmHg) with a standard deviation of 4 (mmHg). Several experiments have been performed in order to characterize and test this system.
Keywords :
biomedical equipment; blood vessels; electro-optical devices; fluorescence; haemorheology; medical signal processing; oxygen; radiation quenching; skin; anaesthetized hamster; blood; control circuit; electro-optical system; fluorescence lifetime; fluorescence quenching; fluorophore; micro-hemo-vessels; microcirculation; porphyrin; real-time oxygen concentration estimation; signal processing; skin; Blood; Circuits; Control systems; Fluorescence; Instruments; Measurement standards; Performance evaluation; Real time systems; Signal processing; Skin; Microciculation; measurament system; oxygen concentration; quenching of the fluorescence;
Conference_Titel :
Engineering in Medicine and Biology Society, 2004. IEMBS '04. 26th Annual International Conference of the IEEE
Conference_Location :
San Francisco, CA
Print_ISBN :
0-7803-8439-3
DOI :
10.1109/IEMBS.2004.1403650
