Title :
Electrical detection of the contact between a microinjection pipette and cells
Author :
Lukkari, M.J. ; Karjalainen, M.I. ; Sarkanen, R. ; Linne, M.-L. ; Jalonen, T.O. ; Kallio, P.J.
Author_Institution :
Inst. of Autom. & Control, Tampere Univ. of Technol., Finland
Abstract :
This work presents the basic design and tests of a device designed for detecting the contact between a microinjection pipette and cell membrane. The device facilitates the automation of the microinjection procedure of living adherent cells. The measurement of the contact is based on measuring the resistance of the pipette. Breakage and clogging of the pipette can be detected with the same measurement.
Keywords :
bioelectric phenomena; biomedical equipment; biomedical measurement; biomembranes; cellular biophysics; electrical contacts; cell membrane; electrical contact detection; living adherent cells; microinjection pipette; micromanipulator; pipette breakage; pipette clogging; resistance measurement; Clamps; Contacts; Current measurement; Electrical resistance measurement; Electrodes; Immune system; Microinjection; Micromanipulators; Pulse measurements; Voltage; Contact; microinjection; micromanipulator;
Conference_Titel :
Engineering in Medicine and Biology Society, 2004. IEMBS '04. 26th Annual International Conference of the IEEE
Conference_Location :
San Francisco, CA
Print_ISBN :
0-7803-8439-3
DOI :
10.1109/IEMBS.2004.1403735
