MEMS-based flow cytometry: microfluidics-based cell identification system by fluorescent imaging

Author

Wu, W.K. ; Liang, C.K. ; Huang, J.Z.

Author_Institution

Dept. of Electr. Eng., Southern Taiwan Univ. of Tech., Taipei, Taiwan

Volume

1

fYear

2004

fDate

1-5 Sept. 2004

Firstpage

2579

Lastpage

2581

Abstract

This study utilizes MEMS technology to realize a novel low-cost microfluidics-based biochip system for flow-type cell handling. Powered by vacuum pump, the microfluidic driving system enables cells to move in order one by one in the biochip by an effect of sheath flow prefocus. Then, cells are guided to a fluorescent inspection region where two detection tasks such as cell image identification and cell counting are conducted. Currently, the glass-based biochip has been manufactured and all the related devices have been well set up in our laboratory. With this proposed prototype system, typical results about cell separation of yeast cell and PC-3 cell are available and their separated images are also presented, respectively.

Keywords

biological techniques; cellular biophysics; fluorescence; microfluidics; MEMS-based flow cytometry; PC-3 cell; cell counting; cell image identification; cell separation; flow-type cell handling; fluorescent imaging; glass-based biochip; microfluidics-based biochip system; microfluidics-based cell identification system; sheath flow prefocus; yeast cell; Charge coupled devices; Data acquisition; Fluid flow control; Fluorescence; Glass manufacturing; Inspection; Mechatronics; Microchannel; Microfluidics; Micromechanical devices; biochip; flow-type cell handling; image identification; microfluidics;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2004. IEMBS '04. 26th Annual International Conference of the IEEE

Conference_Location

San Francisco, CA

Print_ISBN

0-7803-8439-3

Type

conf

DOI

10.1109/IEMBS.2004.1403741

Filename

1403741