Title :
MEMS-based flow cytometry: microfluidics-based cell identification system by fluorescent imaging
Author :
Wu, W.K. ; Liang, C.K. ; Huang, J.Z.
Author_Institution :
Dept. of Electr. Eng., Southern Taiwan Univ. of Tech., Taipei, Taiwan
Abstract :
This study utilizes MEMS technology to realize a novel low-cost microfluidics-based biochip system for flow-type cell handling. Powered by vacuum pump, the microfluidic driving system enables cells to move in order one by one in the biochip by an effect of sheath flow prefocus. Then, cells are guided to a fluorescent inspection region where two detection tasks such as cell image identification and cell counting are conducted. Currently, the glass-based biochip has been manufactured and all the related devices have been well set up in our laboratory. With this proposed prototype system, typical results about cell separation of yeast cell and PC-3 cell are available and their separated images are also presented, respectively.
Keywords :
biological techniques; cellular biophysics; fluorescence; microfluidics; MEMS-based flow cytometry; PC-3 cell; cell counting; cell image identification; cell separation; flow-type cell handling; fluorescent imaging; glass-based biochip; microfluidics-based biochip system; microfluidics-based cell identification system; sheath flow prefocus; yeast cell; Charge coupled devices; Data acquisition; Fluid flow control; Fluorescence; Glass manufacturing; Inspection; Mechatronics; Microchannel; Microfluidics; Micromechanical devices; biochip; flow-type cell handling; image identification; microfluidics;
Conference_Titel :
Engineering in Medicine and Biology Society, 2004. IEMBS '04. 26th Annual International Conference of the IEEE
Conference_Location :
San Francisco, CA
Print_ISBN :
0-7803-8439-3
DOI :
10.1109/IEMBS.2004.1403741
