Electrolytically generated oxygen microgradients for cell culture

Author

Park, J.H. ; Bansal, T. ; Maharbiz, M.M.

Author_Institution

Dept. of Electr. Eng. & Comput. Sci., Michigan Univ., Ann Arbor, MI, USA

Volume

1

fYear

2004

fDate

1-5 Sept. 2004

Firstpage

2683

Lastpage

2686

Abstract

We present a microdevice capable of electrochemically generating user-defined oxygen gradients for use in cell and tissue culture. Electrolytic dissolved oxygen generation at multiple electrodes evolves 1D and 2D oxygen gradients across several millimeters with microscale precision and has the potential to test the effect of localized oxygen doses on a wide range of tissue and cell samples. The developed microgradients are stable for days, enabling experiments of physiologically relevant duration. We present the basic theory of operation and initial results.

Keywords

electrochemical electrodes; electrolysis; micromechanical devices; tissue engineering; bioMEMS; cell culture; electrolytically generated oxygen; microdevice; microgradients; multiple electrodes; oxygen gradients; tissue culture; Assembly; Electrochemical processes; Electrodes; Fabrication; Geometry; Glass; Neoplasms; Oxygen; Resists; Testing; BioMEMS; cell culture; microgradient;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2004. IEMBS '04. 26th Annual International Conference of the IEEE

Conference_Location

San Francisco, CA

Print_ISBN

0-7803-8439-3

Type

conf

DOI

10.1109/IEMBS.2004.1403770

Filename

1403770