Title :
Agarose Gel Investigation of Quantum Dots Conjugated With Short ssDNA
Author :
Tsai-Chin Wu ; Dutta, Maitreyee ; Stroscio, Michael A.
Author_Institution :
Dept. of Bioeng., Univ. of Illinois at Chicago, Chicago, IL, USA
Abstract :
Herein, we investigate the migration distance of quantum-dot-functionalized complexes in electrophoresis. The quantitative study of these moving particles in an electrophoretic environment is modeled using an extended Smoluchowski equation. An extended Smoluchowski equation is proposed to addressed the Dm to Ln(N) plot slope variation issue present in previous work and agreement between experiment and theory is found. The procedures underlying this work then discusses the potential of using agarose electrophoresis as a mean of monitoring the composition of nano-complexes consisting of quantum dots functionalized with differing numbers of DNA molecules.
Keywords :
DNA; biochemistry; biological techniques; electrophoresis; molecular biophysics; nanostructured materials; quantum dots; DNA molecules; agarose electrophoresis; agarose gel investigation; electrophoretic environment; extended Smoluchowski equation; migration distance; moving particles; nanocomplex composition; nanocomplex monitoring; plot slope variation; quantum dot; quantum-dot-functionalized complexes; short ssDNA; DNA; Molecular biophysics; Quantum dots; DNA; Smoluchowski equation; double layer; gel electrophoresis; quantum dots;
Journal_Title :
NanoBioscience, IEEE Transactions on
DOI :
10.1109/TNB.2013.2268540
