Title :
Dynamic visualization of mitochondria in cardiomyocytes with third harmonic generation microscopy
Author :
Barzda, V. ; Greenhalgh, C. ; der Au, A. ; Elmore, S. ; van Beek, J. ; Squier, J.
Author_Institution :
Dept. of Phys., Toronto Univ., Mississauga, Ont., Canada
Abstract :
Dynamic visualization of mitochondria within cardiomyocytes using third harmonic generation microscopy reveals intensity fluctuations similar to those seen in fluorescence microscopy. These changes bring a photobleaching free method for physiological investigations of mitochondria in cardiomyocytes.
Keywords :
biological techniques; cellular biophysics; optical harmonic generation; optical microscopy; optical saturable absorption; cardiomyocytes; dynamic mitochondria visualization; fluorescence microscopy; intensity fluctuations; photobleaching free method; physiological mitochondria investigation; third harmonic generation microscopy; Biological information theory; Cardiology; Fluorescence; Frequency conversion; Microscopy; Optical harmonic generation; Optical imaging; Photobleaching; Ultrafast optics; Visualization;
Conference_Titel :
Lasers and Electro-Optics, 2005. (CLEO). Conference on
Print_ISBN :
1-55752-795-4
DOI :
10.1109/CLEO.2005.202178
