Adhesion of tooth germ cells on various biomaterials

The purpose of this study is to evaluate the interactions of rat tooth germ cells on various biomaterials. The biomaterials used in this study included polyvinyl alcohol (PVA), poly (lactic-co-glycolic acid) (PLGA), poly (ethylene-co-vinyl alcohol) (EVAL), and polyvinylidene fluoride (PVDF). Cells morphology was observed by photomicroscope. Cells growth were separately assayed with 3-(4, 5-dimefhylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) reduction activity. Results indicated that behaviors of tooth germ cells on materials might differ to extent depending on the surface hydrophilicity. On the moderate hydrophilic materials, the behavior and growth of cells were completely flattened and well spread. In contrast, cells on extreme hydrophilic materials were aggregated and unevenly distributed without monolayer formation; cells on extreme hydrophobic material exhibited moderate spreading.